Routine manual stereo microscopes leica microsystems. Leica dm2500 upright fluorescent microscope manuals. The leica sp8 confocal microscope is for live cell imaging with highspeed, multispectral, supersensitivity, superresolution, and fluorescence lifetime capability. Designed to help you maximize efficiency, leica dcm8 unites the advantages of high definition confocal microscopy with interferometry into one versatile, dualcore system.
It is a bundle of a confocal or multiphoton microscope from leica. Laser safety for confocal microscope page 1 of 2 rev. Training guide for leica sp8 confocal multiphoton microscope las af v3. Discover the new lsm 9 family for confocal 4d imaging with high sensitivity and spectral. Leica dmi8 motorized inverted motorized scanning stage lasers. Ultrafast analysis is ensured thanks to oneclick mode selection, sophisticated software and hd confocal scanning without. Raise stage to appropriate height and mount sample. Leica sp5 general training manual sept 2010 robarts. Pcic sp5 confocal microscope user manual, helene javot updated by mamta srivastava 2112017, page 116 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Many pages are copied from internet web pages or from presentations given by leica, zeiss and other companies.
Staining is unnecessary when imaging specimens with the tcs sp8 cars coherent antistokes raman scattering confocal microscope. Beckman institute for advanced science and technology. Fraction technologies supplies microscope stereo, upright, inverted, digital, confocal, infrared red system imaging systems, 2d measurement and 3d profiles. The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials research environment. They cover the normal use and maintenance operations. Vintage confocal microscope microscopes are a unique type of used confocal microscopes.
As a result you acquire optical sections with high contrast and high resolution in x, y and z. Leica mc170 hd or leica mc190 hd user manual introduction 11 we congratulate you on purchasing the leica mc170 hd or leica mc190 hd digital microscope camera by leica microsystems. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. View and download leica sp8 training manual online. See the world premiere of our new confocal platform. View and download leica sp5 confocal user manual online. Introduction to confocal laser scanning microscopy leica. Quick operation manual for leica sp5 confocalmultiphoton. Cars microscope label free imaging leica tcs sp8 cars. On the right hand site under the table are three buttons marked pc microscope, scanner. There is currently no extra training fee for this microscope, however, that may change in the future. Instructions for leica sp2 confocal equipped with visible. This system has five lasers generating nine lines for fluorescence excitation 405. Leica sp2 aobs confocal microscope microscopes, imagers.
Imaging diverse fluorophores in fixed or live samples. Science microscope laboratory consumables leica microscope. Navigator software module creates a fast overview of samples. The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials research. Leica sp5 inverted confocal manual duke light microscopy core. Leica sp8 confocal, sted, flim system microscopy and. The high signaltonoise ratio of the leica hyds helps to render the finest details of any specimen. Consultation with the equipment manager is required before using this microscope. Leica sted and confocal duke light microscopy core facility. They implement the universitys laser safety policy at a practical level. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Jan 11, 2008 basic training video for use of the leica confocal microscope at tufts.
The instructions and additional information relating to the components of the confocal. User manual leica dmi6000 microscope university of arizona. Zeiss axio observer z1 manual single or tile imaging with zen. Aobs, and supersensitive leica hyd hybrid detectors you get a confocal microscope with maximum photon efficiency and gapless spectral detection.
What should the user of the laser scanning microscope observe. Mismatch will lead to loss of intensity and resolution. A high sensitivity, inverted, pointscanning confocal system. In a point scanning confocal system, the microscope lenses focus the laser light on one point in the specimen at a time the. Please browse the internet to learn interactively all about. Sp5 confocal microscope user manual, helene javot updated by mamta srivastava.
Make sure you match the immersion media with the mounting media. The cars technology exploits image contrast arising from characteristic vibrational states of different molecules within a specimen. You should consider how the size of the item will impact the efficiency in which you perform your tasks. Many times, the level of accuracy you will need for your project or study will affect your decision. Check to make sure antivibration table is on power switch in the back of unit 3. Use the confocal controller, or the microscope controller. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes.
This modulare product range can be customized to your needs with ergonomic accessories, special bases and extensive illumination options. The special design of the leica mc170 hd or leica mc190 hd makes it a universal, highly versatile tool for viewing microscopic speci. Leica sp5 confocal user manual date of first issue. Introduction to confocal laser scanning microscopy leica this presentation has been put together as a common effort of urs ziegler, anne greet bittermann, mathias hoechli. Existing users are not allowed to train other users. This microscope does not have a brightfield detector. Leica dmi6000 user manual march 2019 update page 2 leica dmi6000 microscope user policies. The instructions and additional information relating to the components of the confocal system. Ultimate optical design and stability with extreme sensitivity and superb image quality. This video shows how to configure the internal laser power wavelength etc. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix. Leica dm2500 upright fluorescent microscope manuals cell. Fluorescence lifetime gated detection or imaging is available. This user manual specifies names of products or services that are trademarks or registered.
Switch microscope tft to fluo to activate the incident path from default cs in combi. Quick operation manual for leica sp5 confocalmultiphoton microscope. Basics of fluorescence microscopy and the principle of confocal microscopy multidimensional image acquisition with a point scanning confocal microscope advanced applications. Training manual leica tcs sp5 confocal microscope september 2010. Basic training video for use of the leica confocal microscope at tufts. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces.
Olympus ix70 inverted fluorescent microscope manuals. Instructions for using leica dcm3d confocal microscope. Make sure that your sample slides are clean and sealed. Turning on the system 1turn on the fluorescent light source number 1. Usermanual for leica sp8 confocal universitetet i bergen. Buy a microscope leica microscopes microscope central. This confocal is reserved for primary and joint cdb faculty and their personnel. Remove the dust cover from the microscope turn on the mercury lamp if you need to see the fluorescence through the eye pieces. Quick operation manual for leica sp5 confocal multiphoton microscope. Leica is a leader in the field of microscopy and has been providing innovative solutions to the. The confocal microscope and accessories the instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy. Live cell imaging with adaptive focus control afc to automatically maintain focus in timelapse experiments. Leica is a leader in the field of microscopy and has been providing innovative solutions to the educational market, industrial market, and clinical market since 1925. Instructions for the leica sp5 ii laser scanning confocal microscope.
User guide to the ibif leica tcs sp8 mp confocal microscope. National chiao tung university, bio photonics lab, bypraveen kumar. The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser. Confocal laser scanning microscopes scan samples sequentially point by point, or multiple points at once. Enter you name, the date, the time, and the account number in the user log book. Leica sp5 inverted confocal manual duke light microscopy. German company specializing in microscopes and microscopy imaging equipment for medical.
Microscope laboratory consumables leica microscope. Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%. Leica sp5 laser scanning confocal microscope ucsf drc. Microscope central is proud to offer an extensive line of leica microscopes, cameras, and accessories. This manual is the first section of a three part leica tcs sp5 user guide. Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc.
The leica sp5 laser scanning confocal system is used for advanced multichannel fluorescence and livecell applications. Whether you need an entrylevel solution or an advanced analysis and documentation package, leica microsystems provides ergonomicallydesigned manual stereo microscopes for routine tasks. The confocal microscope system for highest demands in multiuser facilities. It presents you with the first steps for activating and commissioning the system and provides important information about the leica application. Reserve the leica sp2 aobs confocal microscope read the user manual. Leica sp2 aobs confocal microscope microscopes, imagers and. Leica tcs sp5 confocal laser scanning microscope user guide. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan. Imaging diverse fluorophores in fixed or live samples temperature, humidity, co2 control spectrally tunable detectors ultrafast resonant scanning reduced photodamage with live samples 3d zstacks, timelapse, multiposition timelapse frapflipphotoactivation, fret ratio or acceptor photobleaching. Starting the lcs software on the leica tcs sp5 confocal microscope. How to use leica confocal microscope sp5 external laser. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented. Leica sp5 scanning confocal cdb equipment resource.
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